Drugging homologous recombination: back to the future
نویسندگان
چکیده
منابع مشابه
Designing E1 Deleted Adenoviral Vector by Homologous Recombination
Adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generat...
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Exchange of strands between homologous DNA molecules is catalyzed by evolutionarily conserved recombinases. These proteins can occur in different quaternary arrangements: rings or helical filaments. Recent results reveal that recombinase function follows from the filamentous form.
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DNA damage, in the form of DNA double-strand breaks, poses a considerable threat to genomic integrity and cell survival. If left unrepaired, a single double-strand break is sufficient to cause cell death and, if repaired inappropriately, a double-strand break may give rise to a potentially oncogenic translocation. Double-strand breaks in genomic DNA may arise accidentally in a number of ways, i...
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Homologous recombination can be divided into three key steps: strand exchange, branch migration and resolution. The identification of a protein complex that catalyses branch migration and Holliday junction resolution argues that the mechanism of homologous recombination is conserved from bacteria to man.
متن کاملan improved homologous recombination method for rapid cloning of the antibody heavy chain gene and its comparison with the homologous recombination and traditional cloning methods
background: the homologous recombination (hr) is one of the conventional cloning methods for the production of recombinant dna. it is a quick method for in vivo dna cloning without using the high cost restriction enzymes. a few modifications in the cloning procedure can increase the efficiency of this method. m aterials and methods: in this study, effect of heating on the rate of the igg1 heavy...
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ژورنال
عنوان ژورنال: Future Medicinal Chemistry
سال: 2018
ISSN: 1756-8919,1756-8927
DOI: 10.4155/fmc-2018-0096